Antioxidant Assays

🧪 Antioxidant Activity Calculator

Calculate % Scavenging, IC50, FRAP value, and more for your extracts/compounds

DPPH

ABTS

FRAP

TEAC

DPPH Radical Scavenging Assay

Absorbance of Control (A_control): Absorbance of Sample (A_sample):

Simple IC50 Estimation (multiple concentrations)

Paste concentrations and % RSA (one per line, e.g. 10,45.2):

10,25\n20,48\n50,72\n100,89

ABTS Radical Cation Assay

Absorbance of Blank: Absorbance of Sample:

FRAP (Ferric Reducing Antioxidant Power)

Δ Absorbance at 593 nm (Sample - Blank): Sample concentration (mg/mL or µg/mL):

Note: This is a simplified version. For accurate mmol Fe²⁺/g, use a proper standard curve in lab.

Trolox Equivalent Antioxidant Capacity (TEAC)

Enter sample response and compare to Trolox standard slope.

Sample response (e.g. % RSA or ΔA): Trolox slope (from standard curve, e.g. response per µmol):