🌡️ DNA/RNA Melting Temperature (Tm) Calculator
Calculate Tm for primers, probes & oligonucleotides
🔬 Sequence Input
⚙️ Calculation Parameters
Common Tm Guidelines:
• PCR Primers: 55–65°C
• Probes: 65–75°C
• Good Tm difference between forward & reverse primer: < 5°C
• PCR Primers: 55–65°C
• Probes: 65–75°C
• Good Tm difference between forward & reverse primer: < 5°C
Wallace Rule (Basic): Tm = 2°C × (A+T) + 4°C × (G+C)